{"product_id":"9780443218682","title":"The Immunological Synapse Part C (Methods in Cell Biology)","description":"\u003cp\u003eThe Immunological Synapse - Part C, Volume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand γδ T cells and evaluate their cytotoxicity.\u003c\/p\u003e","brand":"Academic Press Inc","offers":[{"title":"Default Title","offer_id":49875838402808,"sku":"00000_00000_00000_00000","price":342.71,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0789\/8208\/6904\/files\/9780443218682-1.jpg?v=1783596433","url":"https:\/\/kinokuniya.com.au\/products\/9780443218682","provider":"Books Kinokuniya Australia","version":"1.0","type":"link"}